One more step in solving the Chydorus puzzlea morphological comparison between Chydorus tilhoi Rey & Saint-Jeans, 1969 and Chydorus sphaericus (O.F. Mller, 1776) (Crustacea: Cladocera).

A number of species of Chydorus Leach, 1816 (Crustacea: Cladocera) need improvements in their taxonomy much more than any other genus within the family Chydoridae Dybowsky & Grochowski, 1894 emend. Frey, 1967, which makes the systematics of the genus still a puzzle that lacks several pieces. Here, we redescribe the African species Chydorus tilhoi Rey & Saint-Jeans, 1969 and compare its morphology with that of Chydorus sphaericus (O.F. Mller, 1776). The two taxa might be easily differentiated because C. tilhoi has a single and relatively large major head pore with a wide rim, labral keel elongated with a large spine, and postabdomen with postanal part elongated, narrowing distally and with denticles near its anal margin, organized in groups. These morphological traits are absent in C. sphaericus. Chydorus tilhoi and C. sphaericus also differ in the morphology of the first (Inner Distal Lobe setae), third (exopodite proportion), and fifth (exopodite shape) limbs. Based on the literature and our observations, the limb morphology of C. tilhoi has important similarities with that of C. breviceps, C. nitidulus and C. dentifer, and their translocation to a new genus seems to be a fundamental piece in the puzzle of Chydorus.

Differential interference effects of thermal pollution on the induced defense of different body-sized cladocerans.

Climate warming influences the biological activities of aquatic organisms, including feeding, growth, and reproduction, thereby affecting predator-prey interactions. This study explored the variation in thermal sensitivity of anti-predator responses in two cladoceran species with varying body sizes, Daphnia pulex and Ceriodaphnia cornuta. These species were cultured with or without the fish (Rhodeus ocellatus) kairomone at temperatures of 15, 20, 25, and 30 °C for 15 days. Results revealed that cladocerans of different body sizes exhibited varying responses to fish kairomones in aspects such as individual size, first-brood neonate size, total offspring number, average brood size, growth rate, and reproductive effort. Notably, low temperature differently affected defense responses in cladocerans of different body sizes. Both high and low temperatures moderated the intensity of the kairomone-induced response on body size at maturity. Additionally, low temperature reversed the reducing effect of fish kairomone on the total offspring number, average brood size, and reproductive effort in D. pulex. Conversely, it enhanced the increasing effect of fish kairomone on these parameters in C. cornuta. These results suggest that inducible anti-predator responses in cladocerans are modifiable by temperature. The differential effects of fish kairomones on various cladocerans under temperature influence offer crucial insights for predicting changes in predator-prey interactions within freshwater ecosystems under future climate conditions.

Environmental factors controlling seasonal and spatial variability of zooplankton in thermokarst lakes along a permafrost gradient of Western Siberia.

Humic thermokarst lakes of permafrost peatlands in Western Siberia Lowland (WSL) are major environmental controllers of carbon and nutrient storage in inland waters and greenhouse gases emissions to the atmosphere in the subarctic. In contrast to sizable former research devoted to hydrochemical and hydrobiological (phytoplankton) composition, zooplankton communities of these thermokarst lakes and thaw ponds remain poorly understood, especially along the latitudinal gradient, which is a perfect predictor of permafrost zones. To fill this gap, 69 thermokarst lakes of the WSL were sampled using unprecedented spatial coverage, from continuous to sporadic permafrost zone, in order to assess zooplankton (Cladocera, Copepoda, Rotifera) diversity and abundance across three main open water physiological seasons (spring, summer and autumn). We aimed at assessing the relationship of environmental factors (water column hydrochemistry, nutrients, and phytoplankton parameters) with the abundance and diversity of zooplankton. A total of 74 zooplankton species and taxa were detected, with an average eight taxa per lake/pond. Species richness increased towards the north and reached the maximum in the continuous permafrost zone with 13 species found in this zone only. In contrast, the number of species per waterbody decreased towards the north, which was mainly associated with a decrease in the number of cladocerans. Abundance and diversity of specific zooplankton groups strongly varied across the seasons and permafrost zones. Among the main environmental controllers, Redundancy Analysis revealed that water temperature, lake area, depth, pH, Dissolved Inorganic and Organic Carbon and CO2 concentrations were closely related to zooplankton abundance. Cladocerans were positively related to water temperature during all seasons. Copepods were positively related to depth and lake water pH in all seasons. Rotifers were related to different factors in each season, but were most strongly associated with DOC, depth, CH4, phytoplankton and cladoceran abundance. Under climate warming scenario, considering water temperature increase and permafrost boundary shift northward, one can expect an increase in the diversity and abundance of cladocerans towards the north which can lead to partial disappearance of copepods, especially rare calanoid species.

Mechanisms of harmful effects of Microcystis aeruginosa on a brackish water organism Moina mongolica based on physiological and transcriptomic responses.

To investigate the detrimental impacts of cyanobacterial bloom, specifically Microcystis aeruginosa, on brackish water ecosystems, the study used Moina mongolica, a cladoceran species, as the test organism. In a chronic toxicology experiment, the survival and reproductive rates of M. mongolica were assessed under M. aeruginosa stress. It was observed that the survival rate of M. mongolica fed with M. aeruginosa significantly decreased with time and their reproduction rate dropped to zero, while the control group remained maintained stable and normal reproduction. To further explore the underlying molecular mechanisms of the effects of M. aeruginosa on M. mongolica, we conducted a transcriptomic analysis on newly hatched M. mongolica cultured under different food conditions for 24 h. The results revealed significant expression differences in 572 genes, with 233 genes significantly up-regulated and 339 genes significantly down-regulated. Functional analysis of these differentially expressed genes identified six categories of physiological functional changes, including nutrition and metabolism, oxidative phosphorylation, neuroimmunology, cuticle and molting, reproduction, and programmed cell death. Based on these findings, we outlined the basic mechanisms of microcystin toxicity. The discovery provides critical insights into the mechanisms of Microcystis toxicity on organisms and explores the response mechanisms of cladocerans under the stress of Microcystis.

Resilience of rotifers and cladocerans communities in four reservoirs with eutrophication pollution and lead concentrations in Aguascalientes, Mexico.

Pollution in aquatic ecosystems has been increasing drastically worldwide changing their water quality. Therefore, species must be adapted to these new scenarios. In Aguascalientes City, four representative urban reservoirs contain lead in the water column and extremely high concentrations of sediments. Therefore, an analysis was conducted to evaluate the resilience of zooplankton species to lead exposure in each reservoir using dormant and organisms. Results demonstrated a decrease range from 57.5 to 22.5% in overall diapausing egg hatching rate, while survivorship rate also decreased from 98 to 54% when organisms were exposed to the water of the four reservoirs and increasing lead concentrations. When Pb exposure increased, results showed a global negative effect on both hatching rate (decreasing from 58 to 30% at 0.09 mg L-1) and survivorship levels (decreasing from 100% to 0.07% at 0.09 mg L-1). We provide Species Sensitivity Distribution for both water reservoir dilutions and lead concentration to analyze diapausing eggs hatching and survivorship of offspring in the presence of same polluted conditions or lead of the autochthonous species found in reservoirs. Furthermore, specific analysis with two populations of the cladoceran Moina macrocopa showed clear dissimilar hatching patterns that suggested a different adaptive mechanism. Niagara population shows a hatching rate of approximately 25% in the first two days of reservoir water exposure, while UAA population drastically increased hatching rate to 75% on exposure at day seven. We provide the first record of bioaccumulation in ephippia of M. macrocopa.

Ecotoxicity of the fluoroquinolone antibiotic delafloxacin to the water flea Simocephalus vetulus and its offspring under the influence of calcium modulation.

Delafloxacin (DFX), one of the latest additions to the fluoroquinolone antibiotics, is gaining heightened recognition in human therapy due to its potential antibacterial efficacy in a wide range of applications. Concerns have arisen regarding its presence in the environment and its potential interactions with multivalent metals, such as calcium (Ca). The present study investigated the trans- and multigenerational effects of environmentally projected concentrations of DFX (100-400 µg DFX L-1) on individual- and population-level responses of parental S. vetulus (F0) and its descendants (F1) under normal (26 mg L-1) and high (78 mg L-1) Ca conditions. Exposure of the F0 generation to DFX under the normal Ca condition resulted in reduced juvenile body length (JBL), increased age-specific survival rate (lx), indicating prolonged developmental time, reduced age-specific fecundity rate (mx), and decreased population growth rate (rm). Under the high Ca condition, JBL, mx, and rm were adversely affected. Transgenerational effects of DFX existed, as F1 individuals exhibited persistent suppressions in at least one endpoint under both Ca conditions even after being transferred to a clear medium. Continuous exposure of the F1 generation to DFX had negative impacts on JBL, mx, and rm under the normal Ca condition, and on JBL and rm under the high Ca condition. However, cumulative effects were not observed, suggesting the potential development of tolerance to DFX in the F1 organisms. These findings suggest that DFX is a harmful compound for the non-target model organism S. vetulus and reveal a potential antagonism between DFX and Ca. Nevertheless, the interaction between other (fluoro)quinolones and Ca remains unclear, necessitating further research to establish this phenomenon more comprehensively, including understanding the interaction mechanism in ecotoxicological contexts.

Declining calcium concentration drives shifts toward smaller and less nutritious zooplankton in northern lakes.

Zooplankton community composition of northern lakes is changing due to the interactive effects of climate change and recovery from acidification, yet limited data are available to assess these changes combined. Here, we built a database using archives of temperature, water chemistry and zooplankton data from 60 Scandinavian lakes that represent broad spatial and temporal gradients in key parameters: temperature, calcium (Ca), total phosphorus (TP), total organic carbon (TOC), and pH. Using machine learning techniques, we found that Ca was the most important determinant of the relative abundance of all zooplankton groups studied, while pH was second, and TOC third in importance. Further, we found that Ca is declining in almost all lakes, and we detected a critical Ca threshold in lake water of 1.3 mg L-1 , below which the relative abundance of zooplankton shifts toward dominance of Holopedium gibberum and small cladocerans at the expense of Daphnia and copepods. Our findings suggest that low Ca concentrations may shape zooplankton communities, and that current trajectories of Ca decline could promote widespread changes in pelagic food webs as zooplankton are important trophic links from phytoplankton to fish and different zooplankton species play different roles in this context.

Decomposition of microplastics using copper oxide/bismuth vanadate-based photocatalysts: Insight mechanisms and environmental impacts.

To mitigate marine pollution, we improved the photo-Fenton reaction of modified nanoscale CuO/BiVO4 photocatalysts to resolve the challenge of efficient microplastic degradation in wastewater treatment. Material property analysis and computational results revealed that deposition of CuO onto BiVO4 nanocomposites improved photocatalytic activity by promoting an excess of electrons in CuO and surface charge transfer, resulting in an increased production of e--h+ for ROS generation via H2O2 activation. 1O2 was dominated and identified through quenching experiments, XPS analysis, and EPR. ROS generation increased via H2O2 activation, causing major surface abrasion and increased carbonyl and vinyl indices in microplastics. Treated water had minimal impact on Lycopersicon esculentum Mill. seedling growth but caused considerable mortality in cell lines and Moina macrocopa mortality at greater dosages due to their sensitivity to ions and H2O2 residuals. Overall, this treatment can effectively degrade microplastics, but the dilution of treated water is still needed before being discharged.

Application of Moina mongolica and Daphniopsis tibetana in marine ecotoxicology studies in China: A review.

Moina mongolica and Daphniopsis tibetana are typical saline Cladocera in China that are characterized by a wide salinity range, rapid reproduction, and high-density culture. In this paper, the characteristics and life history of M. mongolica and D. tibetana are reviewed. The application of these two species of Cladocera to ecotoxicology in recent years is also summarized from the aspects of environmental factors and environmental pollutants, including ultraviolet B radiation, temperature, salinity, alkalinity, pH, heavy metals, harmful red tide, pesticides, and persistent organic pollutants. Additionally, the toxicity sensitivity of saline Cladocera in different reproductive statuses and inter-generational embryos is discussed. Finally, the need to enhance knowledge of the molecular genomics, population dynamics, and strategies for protection of saline Cladocera, along with the need for establishment of estuarine and marine environmental monitoring standards are discussed. Overall, this review highlights the potential for using these Cladocera species as indicator organisms for estuarine and marine ecotoxicology studies.

Interactive effects of climate-atmospheric cycling on aquatic communities and ecosystem shifts in mountain lakes of southeastern Tibetan Plateau.

Recent climate warming and atmospheric reactive nitrogen (Nr) deposition are affecting a broad spectrum of physical, ecological and human systems that may be irreversible on a century time scale and have the potential to cause regime shifts in ecological systems. These changes may alter the limnological conditions with important but still unclear effects on lake ecosystems. We present changes in cladoceran with comparisons to diatom assemblages over the past ~200 years from high-resolution, well-dated sediment cores retrieved from six high mountain lakes in the southeastern (SE) margin of the Tibetan Plateau. Our findings suggest that warming and the exponential increase of atmospheric Nr deposition are the major drivers of ecological regime changes. Shifts in cladoceran and diatom communities in high alpine lakes began over a century ago and intensified since 1950 CE, indicating a regional-scale response to anthropogenic climate warming. Zooplankton in the forest lakes showed asynchronous trajectories, with increased Nr deposition as a significant explanatory factor. Forest lakes with higher dissolved organic carbon (DOC) concentrations partially buffered the impacts of Nr deposition with little structural change, while lakes with low DOC display symptoms of resilience loss related to Nr deposition. Biological community compositional turnover in subalpine lakes has shown marked shifts, equivalent to those of low-elevation lakes strongly affected by direct human impacts. This suggests that local effects override climatic forcing and that lake basin features modified by anthropogenic activity act as basin-specific filters of common forcing. Our results indicate that snow and glacial meltwaters along with nutrient enrichment related to climate warming and atmospheric Nr deposition, represent major threats for lake ecosystems, even in remote areas. We reveal that climate and atmospheric contaminants will further impact ecological conditions and alter aquatic food webs in higher altitude biomes if climate and anthropogenic forcing continue.

An integrated transcriptome-microbiome host relationship associated with paraben toxicity in the brackish water flea Diaphanosoma celebensis.

Parabens, a group of alkyl esters of p-hydroxybenzoic acid, have been found in aquatic systems in particular, leading to concerns about their potential impact on ecosystems. This study investigated the effects of three commonly used parabens, methylparaben (MeP), ethylparaben (EtP), and propylparaben (PrP), on the brackish water flea Diaphanosoma celebensis. The results showed that PrP had the most adverse impact on survival rates, followed by EtP and MeP, while MeP and EtP induced significant adverse effects on reproductive performance. A transcriptome analysis revealed significant differential gene expression patterns in response to paraben exposure, with MeP associated with the most significant effects. MeP and EtP exposure produced greater disruption in the microbiota of D. celebensis than did PrP compared with control groups, and we identified eight key microbiota, including Ruegeria and Roseovarius. Correlation analysis between transcriptome and microbiome data revealed key interactions between specific microbiota and host gene expression. Certain microbial taxa were associated with specific genes (e.g. cuticle related genes) and toxicological pathways, shedding light on the complex molecular response and in vivo toxicity effects of parabens. These findings contribute to a deeper understanding of the molecular mechanisms underlying paraben toxicity and highlight the importance of considering the ecological impact of chemical contaminants in aquatic ecosystems.

Toxicant Responses and Culturing Characteristics of Long-Term Laboratory-Reared and Field Populations of Ceriodaphnia dubia.

Ceriodaphnia dubia is a standardized test organism for regulatory toxicity testing of surface waters and commercial chemicals because of its simplicity to culture and responsiveness to toxicants. For testing convenience, C. dubia is often cultured for extended periods in the laboratory with little knowledge of the impact on subsequent generations. Extended laboratory rearing could impact how they respond to stressors and decrease the accuracy of test results. The present study investigated if C. dubia cultured for an extended period were representative of three recently collected field populations by comparing their culturing characteristics and sensitivities to toxicants. For culturing characteristics, the field cultures were more challenging because they had shorter body lengths, fewer neonates, and higher mortality rates than the laboratory culture. Comparative chronic toxicity tests with sodium chloride and the neonicotinoid insecticide thiamethoxam indicated that the laboratory and field organisms did not differ much in their toxicological responses but did differ in the variability of responses (percentage of coefficient of variation). The differences between the laboratory and field cultures found in the present study highlight the challenges of addressing discrepancies between laboratory and field applications in existing standardized methodologies. Environ Toxicol Chem 2024;43:159-169. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Multigenerational effects of elevated temperature on host-microbiota interactions in the marine water flea Diaphanosoma celebensis exposed to micro- and nanoplastics.

Rising ocean temperatures are driving unprecedented changes in global marine ecosystems. Meanwhile, there is growing concern about microplastic and nanoplastic (MNP) contamination, which can endanger marine organisms. Increasing ocean warming (OW) and plastic pollution inevitably cause marine organisms to interact with MNPs, but relevant studies remain sparse. Here, we investigated the interplay between ocean warming and MNP in the marine water flea Diaphanosoma celebensis. We found that combined exposure to MNPs and OW induced reproductive failure in the F2 generation. In particular, the combined effects of OW and MNPs on the F2 generation were associated with key genes related to reproduction and stress response. Moreover, populations of predatory bacteria were significantly larger under OW and MNP conditions during F2 generations, suggesting a potential link between altered microbiota and host fitness. These results were supported by a host transcriptome and microbiota interaction analysis. This research sheds light on the complex interplay between environmental stressors, their multigenerational effects on marine organisms, and the function of the microbiome.

Acute and chronic effects of triclosan on the behavior, physiology, and multigenerational characteristics of the water flea Moina macrocopa.

Triclosan, a chlorinated biphenyl ether is widely used in industrial products and cosmetics due to its antibiotic activity. Although relatively levels of triclosan have been detected in aquatic ecosystems, limited information is available regarding the acute and chronic impacts of triclosan on aquatic invertebrates, especially planktonic crustaceans. In this study, we analyzed the acute (24 h) and chronic (14 days exposure across three generations) effects of different concentrations of triclosan [1/10 of the no observed effect concentration (NOEC), the NOEC, and 1/10 of the LC50] calculated from the 24 h acute toxicity value, on the water flea Moina macrocopa. In the acute exposure experiment, the 1/10 LC50 value of triclosan significantly reduced survival, feeding rate, thoracic limb activity, heart activity, and acetylcholinesterase activity. In response to the 1/10 LC50 value, intracellular reactive oxygen species increased along with elevated levels of malondialdehyde and glutathione. Enzymatic activities of catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase were significantly increased by the 1/10 LC50 value, suggesting active protection of the antioxidant defense system against oxidative stress. Chronic exposure to the 1/10 NOEC and NOEC values revealed multigenerational adverse impacts of triclosan. The second generation was found to be the most sensitive to triclosan, as the NOEC value significantly reduced the survival rate, body length, and the number of neonates per brood, along with a delayed hatching period. Taken together, these results indicate that even sublethal levels of triclosan can have detrimental effects on the water flea population's maintenance through intergenerational toxicity.

Effects of bifenthrin on microcrustaceans grazing behavior on a phytoplankton assemblage dominated by Cyanobacteria.

Insecticides are widely used for pest control and frequently reach aquatic systems, imposing a risk to the biota. In this work, the effect of environmental concentrations of bifenthrin on the grazing capacity of Simocephalus vetulus (Cladocera) and Argyrodiaptomus falcifer (Copepoda) on phytoplankton was evaluated. Fifteen microcrustacean individuals and a natural phytoplankton assemblage dominated by Cyanobacteria were exposed during 46 h to three concentrations of bifenthrin (C0 0 µg L-1, C1 0.02 µg L-1, and C2 0.05 µg L-1). A significant decrease in both microcrustaceans grazing rates on total phytoplankton was observed in C2 compared to C0 and C1. The filtration rate (ml ind-1 h-1) of S. vetulus decreased significantly for the cyanobacteria Anabaenopsis arnoldii, Dolichospermum circinale, and Glaucospira sp. in C2 compared to C0 and C1. The ingestion rate (org ind-1 h-1) of A. falcifer decreased significantly in C1 and C2 compared to C0 only for A. arnoldii. Regarding phytoplankton morphological groups, the filtration rate of S. vetulus decreased in C1 and C2 compared to C0 for Colonies and Coenobiums in C2 concerning C0 and C1 for Filaments and in C2 compared to C0 for Silicified. For A. falcifer, the ingestion rate was reduced in C2 compared to C0 for Silicified, Flagellated, and Sessile. The results showed that bifenthrin affected both microcrustaceans grazing capacity on phytoplankton, especially at the highest insecticide concentration.

Environmentally relevant concentrations of chemically complex shale gas wastewater led to reduced fitness of water fleas (Daphnia carinata): Multiple lines of evidence approach.

Shale gas hydraulic fracturing generates flowback waters that pose a threat to aquatic organisms if released into the environment. In order to prevent adverse effects on aquatic ecosystems, multiple lines of evidence are needed to guide better decisions and management actions. This study employed a multi-disciplinary approach, combining direct toxicity assessment (DTA) on the water flea Daphnia carinata and LC-MS metabolomics analysis to determine the impact of a major ion salinity control (SC) and a cumulative flowback shale gas wastewater (SGW) from a well in the Beetaloo Sub-basin, Northern Territory, Australia. The exposures included a culture water control, simply further referred to as 'control', SC at 1% and 2% (v/v) and SGW at 0.125, 0.25, 0.5, 1% and 2% (v/v). The results showed that reproduction was significantly increased at SGW 0.5%, and significantly decreased when exposed to SC 2%. SGW 2% was found to be acutely toxic for the D. carinata (< 48-h). Second generation (F1) of D. carinata exposed to 0.125-1% SGW generally saw reduced activity in four oxidative biomarkers: glutathione S-transferase, lipid peroxidation, reactive oxygen species, and superoxide dismutase. At the metabolomics level, we observed significant changes in 103 metabolites in Daphnia exposed to both SGW and elevated salinity, in comparison to the control group. These changes indicate a range of metabolic disturbances induced by SGW and salinity, such as lipid metabolism, amino acid metabolism, nucleotide synthesis, energy production, and the biosynthesis of crucial molecules like hormones and pigments. These multiple lines of evidence approach not only highlights the complexities of SGW's impact on aquatic ecosystems but also underscores the importance of informed decision-making and management practices to safeguard the environment and its inhabitants.

Combined effects of nanoplastics and elevated temperature in the freshwater water flea Daphnia magna.

Global warming and nanoplastics (NPs) are critical global issues. Among NPs, one of the most hazardous types of plastics, polystyrene (PS), poses ecotoxicological threats to several freshwater organisms. The degree of toxicity of PS-NPs is strongly influenced by various environmental factors. This study illustrates the combined effects of temperature and PS-NPs on the water flea Daphnia magna. The sensitivity of D. magna to PS-NPs was tested under control (23 °C) and elevated temperatures (28 °C). As a result, increased temperatures influenced the uptake and accumulation of PS-NPs. Co-exposure to both higher temperatures and PS-NPs resulted in a drastic decrease in reproductive performance. The level of oxidative stress was found to have increased in a temperature-dependent manner. Oxidative stress was stimulated by both stressors, leading to increased levels of reactive oxygen species and antioxidant enzyme activity supported by upregulation of antioxidant enzyme-related genes under combined PS-NPs exposure and elevated temperature. In the imbalanced status of intracellular redox, activation of the p38 mitogen-activated protein kinase signaling pathway was induced by exposure to PS-NPs at high temperatures, which supported the decline of the reproductive capacity of D. magna. Therefore, our results suggest that PS-NPs exposure along with an increase in temperature significantly affects physiological processes triggered by damage from oxidative stress, leading to severely inhibited reproduction of D. magna.

Toxicity of benzyl paraben on aquatic as well as terrestrial life.

Parabens are derivatives of alkyl esters of p-hydroxybenzoic acid and come in different classes. These compounds are primarily used as antimicrobial preservative agents in many commercial products, including cosmetics and pharmaceuticals. Accordingly, Benzyl paraben (BeP) is known to be a potential endocrine disruptor. The aim of this study was to determine the toxicity of benzyl paraben (BeP) on aquatic and terrestrial organisms, specifically Scenedesmus sp., Moina macrocopa, and Eisenia fetida. All the organisms were treated with different concentrations of BeP (0.025 mg/L and 1000 mg/L), and LC25, LC50, and LC90 values were used to measure the toxicity levels. Results showed the LC values of BeP for M. macrocopa (3.3 mg/L, 4.7 mg/L, 7.3 mg/L) and E. fetida (173.2 mg/L, 479.8 mg/L, 1062 mg/L), respectively. Toxicity tests on green algae (Scenedesmus sp.) were conducted, the green algae were subjected to various BeP concentration. At 50 mg/L of BeP, cell viability was reduced to 56.2% and the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay indicated 35.4% viable cells. The chlorophyll value and the biochemical parameters of the algal cells were corroborative with the cell viability test. Lethal indices (LC50) for M. macrocopa and E. fetida were evaluated for their toxicity on biochemical properties and were found to be catalase (0.111 mg/L, 0.5 mg/L), lipid peroxidation (0.072 mg/L, 0.056 mg/L), and total protein (0.309 mg/L, 0.314 mg/L), respectively. Overall, this study demonstrated the toxic impact of BeP on non-target aquatic as well as terrestrial species.

Intergenerational toxic effects of 1-methyl-3-octylimidazolium chloride and 1-dodecylpyridinium chloride on the water flea, Moina macrocopa.

Ionic liquids (ILs) are generally considered eco-friendly alternatives to conventional industrial solvents, but they are hard to degrade and easily accumulate in the environment. Therefore, their long-term toxicities are especially vital to estimate their potential risk. However, the chronic toxicities of ILs over generations lacked intensive investigation. In the present work, acute toxicity and chronic toxicity of 1-methyl-3-octylimidazolium chloride ([Omim]Cl) and 1-dodecylpyridinium chloride ([DPy]Cl) were studied on Moina macrocopa with the first exposed generation (F0) and two successive recovery generation (F1 to F2). The acute results showed that both [Omim]Cl and [DPy]Cl exhibited high toxicity to M. macrocopa. The chronic results indicated that the exposure of [Omim]Cl and [DPy]Cl could inhibit the survivorship, body length, and reproduction of M. macrocopa and exhibited a significant dose-related decrease. Furthermore, these two types of ILs presented intergenerational toxicity in the water flea. And the toxic effects of [Omim]Cl disappeared in the recovery tests of F2 generation, while the [DPy]Cl toxic effects continued. Our research suggested a potential risk for the aquatic ecosystem induced by ILs. And the damage done by these chemicals to the aquatic environment is worthy of attention.

Long-term toxicity of chlorpromazine, diclofenac and two lanthanides on three generations of Ceriodaphnia dubia.

Amultigenerational study on Ceriodaphnia dubia was carried out by exposing three subsequent generations to pharmaceuticals chlorpromazine (CPZ) and diclofenac (DCF), and two lanthanide chlorides, gadolinium as GdCl3 and europium as EuCl3. As the treatments, environmentally relevant concentrations were chosen (0.001, 0.01 and 0.1 mg/L for CPZ; 0.1, 1 and 10 mg/L for DCF; 0.425, 4.25 and 42.5 µg/L for Gd and 0.41, 4.1 and 41 µg/L for Eu). Survival, population growth and reproduction success were evaluated at 21 and 30 days of exposure, and the whole observation period lasted 40 days. The least sensitive to all selected substances was the first daphnid generation (F1). Within 21-day exposure, no significant effects of the psychotropic drug CPZ on C. dubia survival were observed in generations F1-F3. The anti-inflammatory drug DCF did not affect survival in the F1 generation; however, it significantly reduced survival in the F3 generation at 1-10 mg/L. Both lanthanides did not affect survival in the F1 and F2 generations of C. dubia but considerably decreased survival in the F3 at 4-42 µg/L. Both pharmaceuticals stimulated the reproduction of C. dubia in the F1 generation, while inhibition occurred at the highest tested concentrations in generations F2 and F3. The inhibitory effect on the reproductive success of lanthanides in the F2 generation resembled that for CPZ but not for DCF. The dynamics of adverse effects during the 21-30-day period revealed that despite increased mortality in the controls (up to 30%), concentrations used in the study minified, in most instances, the survival and aggravated population growth and reproduction success of C. dubia. Our data suggest that C. dubia as a test organism can be used for 21 days in multigenerational investigations, especially when testing close to environmental concentrations. In this respect, the standard C. dubia chronic toxicity assay seems limited since prolonged observations and several generations of daphnids are required to obtain reliable information for the risk assessment of potentially aggressive chemicals.